Genome-broad single nucleotide polymorphism (SNP) markers coupled with allele amount guidance possess came up just like the a powerful equipment getting understanding state-of-the-art qualities in the grown autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48). To date, this process might have been effortlessly applied to the fresh personality of decimal characteristic loci (QTLs) fundamental very heritable characteristics including disease opposition, however, mainly unexplored to possess traits that have state-of-the-art patterns regarding heredity.
Overall performance
In this study, an F1 tetraploid russet mapping population (162 individuals) was evaluated for multiple quantitative traits over two years and two locations to identify QTLs associated with tuber sugar concentration, processing quality, vine maturity, and other high-value agronomic traits. We report the linkage maps for the 12 potato chromosomes and the QTL location with corresponding genetic models and candidate SNPs explaining the highest phenotypic variation for tuber quality and maturity related traits. Significant QTLs for tuber glucose concentration and tuber fry color were detected on chromosomes 4, 5, 6, 10, and 11. Collectively, these QTLs explained between 24 and 46% of the total phenotypic variation for tuber glucose and fry color, respectively. The QTL on chromosome 10 was associated with apoplastic invertases, with ‘Premier Russet’ contributing the favorable allele for fry processing quality. On chromosome 5, minor-effect QTLs for tuber glucose concentration and fry color co-localized with various major-effect QTLs, including vine maturity, growth habit, tuber shape, early blight (Altenaria tenuis), and Verticillium wilt (Verticillium spp.).
Results
Linkage data and QTL mapping when you look at the an excellent russet mapping populace (A05141) using SNP serving guidance successfully identified positive alleles and you may candidate SNPs getting resistance to the brand new buildup from tuber reducing sugar. These unique markers enjoys a top potential for the advance out of tuber handling top quality. Additionally, new finding of different hereditary habits to have faculties with overlapping QTLs from the readiness locus certainly ways a different genetic handle.
Background
Which have an elaborate polyploid family genes the fresh new grown autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48) merchandise novel challenges for linkage mapping and quantitative attribute loci (QTL) study. The new latest development of potato unmarried nucleotide polymorphism (SNP) arrays will bring a couple of genome-broad indicators that can be used during the mapping and you can quantitative trait locus (QTL) investigation [1,2,3,4]bined with the supply of mathematical gadgets that enable the employment off allele dose information (we.e., Boulder escort reviews the number of copies each and every allele within certain polymorphic locus), it is now you’ll be able to so you can somewhat enhance the electricity regarding SNP information in order to detect recombination events in order to use this short article with the QTL mapping [5,6,7]. Thus far, this approach could have been effectively always choose book QTLs and candidate SNPs associated with the late blight resistance [4, 8], inner temperature necrosis , Potato trojan Y , and you will agronomic attributes , but mainly unexplored getting training handling quality or any other highest-worthy of attributes having cutting-edge heredity.
There is certainly a need for unit indicators related to tuber control high quality traits to assist your selection of germplasm to have cultivar invention. Operating quality is basically determined by tuber sugar stuff. The fresh buildup of fructose and you may glucose (cutting sugar) in potato tubers throughout the cold-storage adversely influences the conclusion-play with quality functions. Cutting sugar posts try a complex attribute subject to several genetic and ecological factors [twelve, 13]. The new state-of-the-art genetic architecture from the trait try similar to the interest of several nutrients active in the metabolic pathways hooking up starch description and you will synthesis to help you sugar formation and you can usage [14, 15]. Hereditary mapping research shows that fry processing quality and you may reducing sugar was regarding the thousands of small impression QTLs [16,17,18,19,20]. Soltis-Kalina et al. known a couple significant QTLs to own processor colour on chromosomes step one and six in an effective diploid mapping people. Recently, Braun mais aussi al. advertised two QTLs to have resistance to cooler-triggered sweetening toward chromosomes 4 and you can six off diploid potato. All of these studies have come did into the diploid potato germplasm. But not, after that research is necessary to greatest see the hereditary structures of this type of faculties on tetraploid peak in communities produced from russet-skinned potato variety.
